Genotypic and Phenotypic Detection of Multidrug-Resistant Mycobacterium Tuberculosis among HIV Patients Attending Tuberculosis Reference Centres in Northwest, Nigeria

Abstract

In light of the escalating issue of tuberculosis resistance, it is imperative to consistently evaluate and examine therapeutic strategies. This study focus on genotypic and phenotypic detection of multidrug-resistant Mycobacterium tuberculosis among HIV patients attending tuberculosis reference centres in Northwest, Nigeria. A total of 503 sputum samples were collected aseptically among three referral facilities in Northwest using random sampling method. Molecular drug susceptibility testing was performed using Geno Type® MTBD Rplus assay. The overall prevalence was 4.3 %. The highest prevalence in relation to state was observed from Katsina (22.2 %) and the lowest was from Sokoto (3.6 %). The highest prevalence was observed among male patients (4.8%) while the female patients (3.4%). Age 66-70 yrs recorded the highest prevalence (100 %) and the lowest was 36-40 yrs and 41-45yrs (4.4 %). The highest resistance patterns were isolates from Kebbi were resistance to INH+ RIF+ FLQ (50.0 %) and the lowest was from Kaduna INH+ AMG (20.0 %). Routine TB testing among HIV patients must be improved to guide co-management