Evaluation of Inflammatory Biomarker IL-8 in Helicobacter Pylori-Associated Gastritis

Abstract

Helicobacter pylori gastritis has been recognized as a significant risk factor for the development of gastric cancer. This study aimed to evaluate the role of interleukin-8 (IL-8) in Helicobacter pylori-Associated Gastritis disease progression. A total of 65 participants in the present study 35(58%)with symptoms of gastritis, were diagnosed and included in the study, along with 25 healthy controls. Gastric biopsy samples (mucus layer) were collected, and IL-8 concentrations were measured using an ELISA assay (Solarbio Human IL-8 ELISA Kit). Absorbance was recorded using a Fisher Scientific ELISA plate reader. Patients with gastritis who tested positive for Helicobacter pylori by PCR analysis—using glmM-specific primers—showed significantly elevated levels of IL-8 compared to healthy controls (17.78 pg/mL vs. 9.05 pg/mL, p < 0.001). These findings demonstrate a marked increase in IL-8 levels in patients with H. pylori-induced gastritis, underscoring its critical role in mediating inflammatory responses and contributing to disease pathogenesis.