International Journal of Research and Scientific Innovation (IJRSI)
Isolation and Characterization of Cellulose-Producing Bacteria from Agricultural Wastes
Published February 18, 2026 • Vol. 13, Issue 1, pp. 2701–2721Open Access
DOI: 10.51244/IJRSI.2026.13010233
Abstract
The aim of the present study is to demonstrate the isolation, identification and screening of bacteria with high cellulase activity from soil samples. Cellulase degrading bacteria were isolated from soil sample using serial dilution and pour plate method. It indicated that favourable fermentation conditions and the selection of a suitable growth medium played a key role in the production of cellulase from newly isolated Cellulase sp. Due to its particular characteristics this enzyme will be used in saccharification process for bioethanol production from plant biomasses. An accurate and precise method for the assay of cellulase activity in soil was developed. It involves determination of the reducing sugars produced when a soil sample is incubated with acetate buffer, carboxy methyl cellulase (CMC), and toluene.
Keywords: Cellulose, enzymes, saccharification, Agricultural Wastes
| Journal | International Journal of Research and Scientific Innovation (IJRSI) |
|---|---|
| ISSN | 2321-2705 |
| Volume / Issue | Volume 13, Issue 1 |
| Pages | 2701–2721 |
| Publication date | February 18, 2026 |
| DOI | 10.51244/IJRSI.2026.13010233 |
| Publisher | RSIS International |
| License | Open Access |
How to cite this article
Dr. D. Sripriya (2026). Isolation and Characterization of Cellulose-Producing Bacteria from Agricultural Wastes. International Journal of Research and Scientific Innovation (IJRSI), 13(1), 2701-2721. https://doi.org/10.51244/IJRSI.2026.13010233
BibTeX
@article{Dr2026,
title = {Isolation and Characterization of Cellulose-Producing Bacteria from Agricultural Wastes},
author = {Dr. D. Sripriya},
journal = {International Journal of Research and Scientific Innovation (IJRSI)},
volume = {13},
number = {1},
pages = {2701--2721},
year = {2026},
doi = {10.51244/IJRSI.2026.13010233},
publisher = {RSIS International}
}