Extraction of Gelatin from Cattle Hides by Using Acetic Acid

Abstract

Gelatin is a natural substance made by partially hydrolyzing collagen-rich materials that are present in connective tissues, animal skin, and bones. The objective of this investigation was to ascertain the yield and physicochemical characteristics of gelatin that was extracted from cattle hides that had been exposed to varying concentrations of acetic acid. To attain the highest extraction efficiency, pretreatments are typically performed before the gelatin extraction procedure. Fresh hides of cattle were gathered from a nearby slaughterhouse. In this study, cattle hides treated with 1% acetic acid were categorized as AA-1 group, 3% acetic acid treated hides as AA-3 group, and 5% acetic acid treated hides as AA-5 group with three replicates in each group or treatment. A significant difference (p<0.001) in acetic acid concentration was observed in yield, gel strength/ Bloom, viscosity but did not differ significantly (p>0.05) for moisture, crude protein, crude fat, ash, pH and color (L*, a* and b* values) of the gelatin extracted from cattle hides. Yield of gelatin was highest in AA-5 group (16.98%), followed by AA-3 group (13.99%) and lowest in AA-1 group (11.57%). The highest bloom was observed in AA-3 group (205.52g), followed by AA-5 group (195.52g) and lowest in AA-1 group (154.90g). Bloom increased almost 25.00% in case of AA-3 group compared to AA-1 group. The physiochemical and technological properties of gelatin extracted from cattle hides indicate that high quality gelatin can be extracted from cattle hides after acid pretreatment for application in a variety of fields.