International Journal of Research and Innovation in Applied Science (IJRIAS)
Analytical Method Development and Validation for the Determination of Ethylene Glycol and Diethylene Glycol in Herbal Cough Syrup by Gas Chromatography
Published June 1, 2026 • Vol. 11, Issue 5, pp. 1019–1033Open Access
DOI: 10.51584/IJRIAS.2026.11050089
Abstract
Ethylene Glycol (EG) and Diethylene Glycol (DEG) are potential contaminants in liquid oral formulations, posing severe toxicological risks. Regulatory agencies mandate stringent controls on their permissible limits. This study outlines the analytical method verification of a gas chromatography (GC) procedure for the quantitative determination of EG and DEG in a polyherbal cough syrup. The method employs a DB-624 capillary column and flame ionization detection (FID), using ethanol as the diluent. System suitability, precision, resolution, and tailing factor criteria were established and verified. The method was confirmed to be accurate, precise, and suitable for routine use in quality control.
Keywords: Ethylene Glycol, Diethylene Glycol, Gas Chromatography
| Journal | International Journal of Research and Innovation in Applied Science (IJRIAS) |
|---|---|
| ISSN | 2454-6194 |
| Volume / Issue | Volume 11, Issue 5 |
| Pages | 1019–1033 |
| Publication date | June 1, 2026 |
| DOI | 10.51584/IJRIAS.2026.11050089 |
| Publisher | RSIS International |
| License | Open Access |
How to cite this article
Ankit Sharma, & Subrahmanya Kumar Kukkupuni (2026). Analytical Method Development and Validation for the Determination of Ethylene Glycol and Diethylene Glycol in Herbal Cough Syrup by Gas Chromatography. International Journal of Research and Innovation in Applied Science (IJRIAS), 11(5), 1019-1033. https://doi.org/10.51584/IJRIAS.2026.11050089
BibTeX
@article{Ankit2026,
title = {Analytical Method Development and Validation for the Determination of Ethylene Glycol and Diethylene Glycol in Herbal Cough Syrup by Gas Chromatography},
author = {Ankit Sharma and Subrahmanya Kumar Kukkupuni},
journal = {International Journal of Research and Innovation in Applied Science (IJRIAS)},
volume = {11},
number = {5},
pages = {1019--1033},
year = {2026},
doi = {10.51584/IJRIAS.2026.11050089},
publisher = {RSIS International}
}