Biochemical Analysis of Lentil Seed and Plant Seedling Infected with Fusarium Oxysporum and Rhizoctonia Solani

Abstract

The present research work entitled biochemical analysis of lentil seed and plant seedling infected with fusarium oxysporum and rhizoctonia solani was conducted during Rabi season 2024-25 at the laboratory of modi institute of technology,Kota Rajasthan. The counter parts (Like leaf,stem,fruite and seeds) of lentil were selected for the study. The experiment was conducted using a Completely Randomized Design (CRD) with three replications to evaluate these parts.
Lentil (Lens culinaris) is an important legume crop grown around the world, but its yield often decreases due to fungal infections from Rhizoctonia solani and Fusarium oxysporum, which lead to root rot and damping-off diseases. The biochemical changes that these infections cause in lentil seeds and seedlings are examined in this study. The levels of total protein, phenolic content, antioxidant enzyme activity (peroxidase, catalase, and superoxide dismutase), and lipid peroxidation were among the biochemical markers that were examined in both infected and healthy samples. The results showed that defense-related enzyme activity was noticeably increased in infected plants and oxidative stress indicators, indicating an activated defensive mechanism. This response suggests that lentil plants have developed a robust defense system to combat the effects of rot and damping-off diseases. Further investigation into the specific pathways involved in these biochemical responses could provide valuable insights for improving disease resistance in lentil crops.
Additionally, variations in the accumulation of secondary metabolites were noted, highlighting their possible function in plant defense. The metabolic reactions of lentils to fungal diseases are better understood thanks to this study, which could also help create efficient disease control plans. Using standard quantitative techniques of biochemical analysis, important components of lentil seeds, such as lipid, starch, protein, phenol, and related enzymes, such as α- and β-amylase, polyphenol oxidase, protease, and lipase, were examined in the seeds infected with Rhizoctonia solani and Fusarium oxysporum.