Optimization and Characterization of Biosurfactant Produced by Pseudomonas Aeruginosa

Abstract

Biosurfactant are amphiphilic molecule synthesized by variety of microorganisms as secondary metabolites. Due to non toxicity, biodegradability and eco-friendly nature, biosurfactant can be utilized in various sectors. Previous research studies reported that biosurfactant possess antimicrobial activity against human pathogens. On the circumstances of the increasing market of biosurfactant all over the world, the present study focused on Optimization and characterization of biosurfactant produced by Pseudomonas aeuginosa. Optimization of production media and physiochemical parameters for efficient biosurfctant production was carried out using MSM broth supplemented with 2% soyabean oil. Statistical analysis was carried out. Biosurfactant was characterized on the basis of TLC and Mass spectrometry. The results revealed that soyabean oil as carbon source, KNO3 as nitrogen source, aeration rate of 25vvm, agitation rate of 200 rpm, pH 8, 144 hrs. incubation periods, inoculum size 4% and temperature 30 ̊C was found to be statistically significant for the production of rhamnolipid by Pseudomonas aeruginosa. The finding of thin layer Chromatography showed two spots on silica gel plate after drying. The Rf value was found to be 0.53 and 0.67 respectively confirming presence of mono and di- rhamnolipid. The mass spectra showed the presence of both potassium adducts (M+K)+ ions sodium adducts (M+Na)+ ions confirming the presence of rhamnolipid. Hence, Pseudomonas aeruginosa isolated from oil contaminated soil could be utilized for rhamnolipid production.