L-Proline Determination at Glassy Carbon Electrode

Abstract

Voltammetric determination of L-Proline (L-Pro) using Cyclic voltammetry and Differential pulse voltammetry were recorded at glassy carbon electrode within the potential window 0.0 V and 2.4 V at the physiological pH. The reference and counter electrode used were Ag/AgCl and Pt wire, respectively.The effect of supporting electrolyte and concentration of electro active species on the interaction were also studied.The results demonstrate that the catalytic oxidation of L-Pro is diffusion-controlled and irreversible Under optimized conditions, the calibration curve for L-Pro concentration was linear in 〖3.5×10 〗^(-8) M to 〖1.5×10 〗^(-3) M with a low Limit of Quantification of 〖9.79×10 〗^(-9) M and a Limit of detection of 〖3.23×10 〗^(-8) M. It is shown that the prepared sensor provides a sensitive and rapid strategy for the detection of L-Proline.