Genetic Diversity Analysis of Pisum Sativum Accessions Using SDS-PAGE Protein Profiling and Statistical Approaches

Abstract

Molecular characterization using protein profiling is a crucial technique for understanding genetic variability among plant accessions. The present study investigates the genetic diversity among five Pisum sativum accessions using protein banding patterns, similarity indices, and statistical analyses. SDS-PAGE was employed to assess the presence or absence of protein bands with different molecular weights. The protein bands were analyzed based on their molecular weights (kDa), and the presence or absence of specific bands was recorded across different accessions. The similarity index was calculated to assess the genetic relationships among the accessions. Our findings provide valuable insights into the genetic diversity of Pisum sativum accessions, which may aid in breeding programs and disease resistance studies. A paired t-test and Pearson correlation matrix were used to analyze statistical significance and genetic relationships. The results indicate both conserved and polymorphic protein bands, highlighting genetic variation among the accessions. The paired t-test showed no statistically significant differences between accessions, while the Pearson correlation analysis suggested varying levels of genetic similarity. These findings provide valuable insights for breeding programs aimed at improving disease resistance and adaptability in Pisum sativum.